Tissues and organs that are developed in the lab must not only look like the tissue and organs they will replace, they must also function like them. Once cells are seeded onto scaffolds, mechanical stimulation can be used to enhance differentiation and maturation of the stem cells into a functional load-bearing tissue such as bone, cartilage and tendon. Different types of mechanical stimulation, such as compression, tension and shear, may be applied in constant or changing rates and magnitudes to enhance functional tissue regeneration. We test the role of tension, compression and hydrostatic loading upon stem cell differentiation using custom-made and commercially available devices.
Novel bioreactor for the tensile stimulation of cell-matrix constructs to enhance tenogenic/ligamentous differentiation in vitro developed in cooperation with the Bose corporation.
- Hang Lin
- Peter G Alexander
- Haruyo Yagi
- Riccardo Gottardi