Pathogenesis: OA is the most common type of arthritis. It is estimated that 26.9 million Americans 25 years old or older have clinical OA of some joints, with a higher percentage of affliction in the older population. The etiology of OA is not completely understood; however, injury, age, and genetics have been considered among the risk factors. We have developed a model of post-traumatic osteoarthritis employing a spring-loaded delivery system. Using this system we are investigating various pathogenic mechanisms including epigenetic changes that alter the phenotype of cells following trauma.
Cell viability in articular cartilage after traumatic injury. 5mm x 2mm plugs of adult bovine articular cartilage from the patello-femoral groove of hind stifles were isolated and subjected to a single impact with an average load 36MPa. Samples were bisected transversely 24 hours later and stained with calcein AM (green, for living cells), ethidium homodimer (red, for dead cells) using the Live/Dead assay (Invitrogen).
- Peter G Alexander