Once OA has progressed to a point where pain management is no longer effective, surgical intervention is often employed. Techniques implant are currently use current surgical therapeutic procedures to cartilage repair such as microfracture, mosaioplasty, and autologous chondrocyte are clinically useful In that these procedures can result in improvement of clinical symptoms, such as pain relief, but none of the current treatment options has regenerated long-lasting hyaline cartilage repair tissue yet A potential resolution of this disease state is the regeneration of cartilage tissue using autologous MSCs. Combining the insights gleaned from research in cartilage development, stem cell biology, scaffold design, cell signaling mechanisms, we are developing chondral and osteochondral constructsand testing them in vivo. Recent work in our laboratory strongly suggests that nanofibrous scaffolds fabricated using biodegradable polymers are promising biomaterials for cartilage tissue engineering. Preliminary studies using cell-seeded nanofibrous constructs in a rabbit and mini-pig femoral condyle defect models have yielded significant repair of the articular cartilage.
Post-implantation of a cellular nanofibrous scaffold placed in an iatrogenic defect in the medial femoral condyle of a white new Zealand rabbit. PCL-based nanofibrous scaffolds were combined with allogeneic rabbit bone marrow-derived MSCs by centrifugation and cultured in defined chondrogenic medium supplemented with TGFbeta3 (10ng/ml) for 4 weeks in a HARV reactor. At the time of surgery, the medial femoral condyle was exposed by a medial para-patellar incision and lateral displacement of the patella. A 5.2 mm plug was isolated from the chondrogenic BMD-MSC/PCL nanpfibrous mass and press-fit into a 5mm diameter full-thickness osteochondral defect.
- Hang Lin
- Peter G Alexander